Authorities say the semi-truck was hauling the bees from California to North Dakota when the driver veered off the shoulder, tipping more than 400 hive boxes and honey.
Authorities reportedly began receiving 911 calls late Sunday afternoon.
Fremont County Sheriff deputies say several workers were stung during the first few hours of the cleanup Sunday.
According to KPVI, officials had to spray fire foam on the truck and bees before responders could join the effort. The spill, which also unleashed a torrent of honey,reportedly required crews from numerous agencies.
Some observers told The Post-Register they saw a strange black cloud and heard a roaring noise above the spill area before realizing it was a massive swarm of bees.
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Crews worked all day Monday before removing all the honey from the roadway, though deputies say a significant number of bees were still buzzing